PROFILE OF HIGHER FATTY ACIDS OF BARLEY SEEDS AFTER PROCESSING BY ELECTROMAGNETIC WAVES OF THE MICROWAVE RANGE 

Soboleva Ol'ga Mikhaylovna, Candidate of biological sciences, associate professor, sub-department of microbiology, immunology and virology, Kemerovo State Medical University (22A, Voroshilova street, Kemerovo, Russia), E-mail: meer@yandex.ru
Kondratenko Ekaterina Petrovna, Doctor of agricultural sciences, professor, sub-department of agronomy, breeding and seed production, Kuzbass State Agricultural Academy (5, Markovtseva street, Kemerovo, Russia), E-mail: meer@yandex.ru
Sukhikh Andrey Sergeevich, Candidate of pharmaceutical sciences, associate professor, senior researcher, Central research laboratory, Kemerovo State Medical University (22A, Voroshilova street, Kemerovo, Russia), E-mail: Suhih_as@list.ru 

633.16: 537.87 

DOI

10.21685/2307-9150-2019-4-1 

Background. The mechanism of action of electrophysical factors stimulating plant seeds has not yet been studied. The nature of the influence of the electromagnetic field of ultrahigh frequency on the profile of higher fatty acids in chloroform extract of spring barley seedlings was investigated.
Materials and methods. The object of research was sprouts of spring barley varieties Nikita. Variants of the experiment: control, without treatment; electromagnetic irradiation of dry seeds with a power of 0,42 kW, a frequency of 2,45 GHz, with an exposure of 11 seconds. After germination, the sample was extracted from all anatomical parts of the seedlings with a mixture of chloroform and n-hexane, in which the fatty acid content was determined by gas chromatography.
Results. Was organ specificity: in the leaves and roots prevalent among limiting fatty acids are palmitic, stearic, behenic and lignocerine acid in the endosperm, in the shell of the grain – palmitic and stearic acids. Oleic and linoleic acids predominate among unsaturated fatty acids in all organs of the seedling. The fatty acids profile of leaf and root lipids is saturated, and the endosperm and bran are unsaturated. After exposure to EMF microwave amplifies the formation of long-chain fatty acids; there is a synthesis de novo tricosanoic, eicosenoic acids, isomer of linoleic acid (in the leaves), myristoleic, nervonic acids and isomer of linoleic acid (in roots), myristoleic, eicosadienoic acids (in the endosperm), margarine, heneicosanoic, nervonic, eicosadienoic acids (in the shells of the grain).
Conclusions. New data on the mechanism of action of the electromagnetic field of ultrahigh frequency on seeds and seedlings of cereals obtained. The important role of higher fatty acids in the protective mechanisms of plant cells in response to electromagnetic action is assumed. 

Key words

barley, seedlings, electromagnetic microwave field, fatty acids, gas chromatography with mass spectrometric detection 

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